An Oxygen Sensitive α-Fe2O3–Au Diode

An oxygen-sensitive toxin–antitoxin system

The Hha and TomB proteins from Escherichia coli form an oxygen-dependent toxin-antitoxin (TA) system. Here we show that YmoB, the Yersinia orthologue of TomB, and its single cysteine variant [C117S]YmoB can replace TomB as antitoxins in E. coli. In contrast to other TA systems, [C117S]YmoB transiently interacts with Hha (rather than forming a stable complex) and enhances the spontaneous oxidati...

Tunable Diode Laser Absorption Spectroscopy of Oxygen

An Output-Sensitive Algorithm for Computing Weighted α-Complexes

An α-complex is a subcomplex of the Delaunay triangulation of a point set P ⊂ R that is topologically equivalent to the union of balls of radius α centered at the points of P . In this paper, we give an output-sensitive algorithm to compute α-complexes of n-point sets in constant dimensions, whose running time is O(f log n log αs ), where s is the smallest pairwise distance and f is the number ...

An ESIPT fluorescent probe sensitive to protein α-helix structures.

A large majority of membrane proteins have one or more transmembrane regions consisting of α-helices. Membrane protein levels differ from one type of cell to another, and the expression of membrane proteins also changes from normal to diseased cells. For example, prostate cancer cells have been reported to have downregulated expression of membrane proteins, including zinc transporters, compared...

Phage antibodies against an unstable hapten: oxygen sensitive reduced flavin.

It is difficult to raise antibodies against haptens and antigens that are unstable under the physiological conditions of the serum. Here we have used a phage antibody library to isolate antibody fragments against oxygen sensitive reduced flavin, by selection of the phage under anaerobic and reducing conditions at pH 5 and a pre-elution step with the oxidized flavin. The binding of the reduced h...